giemsa stain procedure for blood smear

Prepare the Giemsa working solution before staining blood film and use it within 15 minutes of preparation. WebThe diluted blood is discharged onto the hemacy- WrightGiemsa Stain Commercially prepared WrightGiemsa stains are available and make the staining procedure relatively simple. 0000005451 00000 n The same laboratory Data We are trying our best to make this site user-friendly and resourceful with timely/updated information about each pathogen, disease caused by them, pathogenesis, and laboratory diagnosis. Then, place another drop of blood at the clear)Tj ET BT 116.043 486.971 TD (end and use the edge of the smearing slide to spread the drop out to about a 1 cm)Tj ET BT 116.043 471.131 TD (circle. 0000001316 00000 n The Giemsa stain is positive and is usually confirmed by the traditional staining method. February 27, 2023. Wrights, May-Grunwald-Giemsa, rapid stains). WebTechnical Procedure Immersion Staining Protocol 1. It is the recommended and most reliable procedure for staining thick and thin blood films from the blood sample of the patient, for precise identification of the causative malaria species. 0000040229 00000 n It should)Tj ET BT 116.043 142.083 TD (take about one second to smear the drop. Since good quality control smears are not available commercially, they may be prepared from a patients blood and stored for future use in the following manner: DPDx is an educational resource designed for health professionals and laboratory scientists. 0000007151 00000 n The technique for making)Tj ET BT 98.762 508.332 TD (and storing dried blood samples is given in the section \322Dried Blood Samples\323. )Tj ET BT 98.762 301.207 TD (3. She has a background in Immunology and Microbiology (MSc./BSc.). Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website. Dark C. Protected away for moisture D. Stored in a wet box 8. Do not push the blood by having it ahead of the smearing slide! Careful observation, however, will reveal that many of these forms have a small, rod-shaped kinetoplast, characteristics of Leishmania amastigotes. These cookies allow us to count visits and traffic sources so we can measure and improve the performance of our site. Immersion oil can be placed directly on the)Tj ET BT 116.043 152.643 TD (smear for observing under 1000x. 4. However, Giemsa requires longer staining time (15 minutes) than NMB. Malaria parasites have a red or pink nucleus and blue cytoplasm. God bless you. Azure and methylene blue, a basic dye binds to the acid nucleus producing blue-purple color. Centers for Disease Control and Prevention. To describe the procedure for quality control (QC) assessment of stock solutions of Giemsa stain and of MM-SOP-07 (Giemsa staining of malaria blood films) for both rapid (10%) and slow (3%) stains. Allow the film to air dry thoroughly for several hours or overnight. document.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); This site uses Akismet to reduce spam. These cookies may also be used for advertising purposes by these third parties. Just a very few mL should be necessary to reach the)Tj ET BT 98.762 518.892 TD (required pH. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (A high-quality Giemsa should be used. Ideally it should be opposite. 0000009735 00000 n Only mammals have erythrocytes that)Tj ET BT 116.043 534.732 TD (lack a nucleus. Giemsa stain is a type of Romanowsky stain, named after Gustav Giemsa, a German chemist who created a dye solution. Then, add 250ml of glycerin to the solution, slowly. Fix smears for 5-10 minutes with methanol. Giemsa is used to identify the mast cells and stains the fungus Histoplasma, and Chlamydia bacteria. )Tj ET BT 98.762 311.767 TD (Slide boxes. WebAbstract Wright-Giemsa staining is a common procedure that is performed routinely in hematology laboratories. Most of ours were hand-me-downs from retiring faculty over the)Tj ET BT 98.762 200.405 TD (years. It can be used if rapid results are needed, but should be followed up when possible with a confirmatory Giemsa stain, so that Schffners dots can be demonstrated. Note: As alternates to this 45-60 minutes in 2.5% Giemsa stain, the smears could be stained for shorter times in more concentrated stains. Calcofluor white staining uses fluorescent dyes to stain the chitin and cellulose in the fungi, plants, and algae cell walls. Giemsa stain was a name adopted from a Germany Chemist scientist, for his application of a combination of reagents in demonstrating the presence of parasites in malaria. Put into a 500 ml brown bottle the glass beads and the other ingredients, in the order listed. This video describes the procedure of Alizarin Red S Staining for osteogenesis. 0000008752 00000 n As a starting point, we used the standard protocol from the manufacturer on blood smears. 0000001897 00000 n WebThe Giemsa stain is used as the gold standard for the diagnosis of malaria on blood smears. Staining Solution 1. Smears are kept after dipping in alcohol in a bag with silica gel. Made with by Sagar Aryal. Sales Office- Yesssworks S14, Pinnacle Business Park M.I.D.C, Andheri East, Mumbai, 400093 (Maharashtra) INDIA. The Cytoplasm and cytoplasmic granules of blood cells appear red in color while the nucleus appears blue-purple in color. Key areas of my work lies in Bacteriology, especially in Antimicrobial resistance. Also notice the high numbers of myeloblasts in the smear. Macsen Labs is a manufacturer and supplier of high-quality Giemsa Stain. Romanowsky stains are applied in the differentiation of cells, pathological examinations of samples like blood and bone marrow films and demonstration of parasites e.g malaria. 0000048353 00000 n Corporate Headquarters- 303, Shivam Residency, Durga Nursery Road, Udaipur - 313001 (Rajasthan) INDIA. Giemsa stain is a type Romanowsky stain that stains nuclei and cells. What is the difference between Leishman stain and Giemsa stain? Briefly dip the slide in and out to wash it. Giemsa Stain is used in malaria diagnosis. Filter the solution and leave it to stand for about 1-2 months before use. 7 days later the peripheral blood smear Giemsa-Wright staining was performed (C, arrowheads indicate the megaloblastic RBCs found only in the iron supplementation group) and the spleens, femurs and tibias were shown (D). )Tj ET BT 98.762 375.609 TD (2. When the fixing parameters were established, the Wright-Giemsa staining procedure was used. If two smears are made per slide, be sure to flip over the spreader to use the)Tj ET BT 116.043 662.175 TD (other edge for the second smear produced. Recommended for detection and identification of blood parasites. )Tj ET BT 98.762 168.724 TD (Silica gel is from Sigma \(S7500\) that we buy in the 1 kg can. Giemsa stain is a differential stain that is used to variably stain the various components of the cells and it can be used to study the adherence of pathogenic It is also used in Wolbachs tissue stain i.e staining hematopoietictissueand for the identification of bacteria and rickettsia. This immunogold-silver staining method was used to quantify T- and B-lymphocytes and natural killer cells in buffy coat smears of normal adult blood. Prewarm the deionized water and slowly add the Triton X-100, swirling to mix. Based on this study, a 5% Giemsa solution is recommended for the staining procedure. These are)Tj ET BT 98.762 295.927 TD (obtained from Carolina Biological Supply \(Carolina Blue Boxes, #HT-63-4200\) \). The Wright-Giemsa-stained impression smear illustrates a few background macrophages and numerous tiny 2 to 3 amastigotes of Leishmania. 2023 Microbe Notes. Keep both chemicals in a locked cabinet or cupboard when they are not in use. Giemsa stain is used in staining blood cells and bacteria that is improved by stabilizing the dye solution with glycerol and is allowed for staining of cells for microscopy purposes. Giemsa stain is the most reliable method for staining thick and thin blood films. WebTerm used to identify immature RBC with large amounts of RNA that precipitate as large chunks or aggregates when the blood is incubated with an intravital dye, such as new methylene blue. )Tj ET BT 116.043 269.526 TD (See the drawing below. JTM708-1, a 500 mL bottle. Storage of unstained slides Under the microscope, this specific result comes out when bacteria, cell organelles, and parasites are distinguished on the basis of morphology and color. Wash by briefly dipping the slide in and out of a Coplin jar of buffered water (one or two dips). Make as many thin smears as possible, preferably within one hour after the blood was drawn from the patient. Choose a patient blood specimen, anticoagulated with EDTA, that has enough parasites so that at least one is found in every 2 to 3 fields. Then stain with diluted Giemsa stain in a Coplin jar. Abcam offers > 1,000 assay kits cited in > 3,500 publications. We do not claim or suggest/advise any medical, therapeutic, health or nutritional benefits of Giemsa Stain. Aggregate reticulocytes correspond to polychromatophilic RBC in a Romanowsky-stained blood smear (e.g. They can then be placed into a plastic slide)Tj ET BT 116.043 295.927 TD (box for complete drying. WebImpression smears (touch preps) can be made (& fixed/stained) locally or at CDC Histopathology slides: - made by local path staff (include H&E and Giemsa, as well as special stains for other microbes) - send slides (esp. )Tj ET BT 98.762 152.643 TD (Zip-lock plastic bags should be the ones used for freezer storage. 0000099606 00000 n With extensive higher education teaching and research experience in Biomedical studies, metagenomic studies, and drug resistance, Faith is currently integrating her Biomedical experience in nanotechnology and cancer theranostics. Putting two smears per slide saves on weight \(glass is heavy\) for field trips,)Tj ET BT 116.043 396.729 TD (and storage space. Giemsa stain (3 ml) is diluted with buffered distilled water (100 ml) and is the stain of choice for A little practice will tell the amount of buffer to add. )Tj ET BT 98.762 248.166 TD (Coplin jars. ), 6 (3.4%) false negatives I am looking for information on the Green Crystals of Death. Anybody? Giemsa stain is a popular microscopic stain that is used in hematology, histology, cytology, and bacteriology. WebBlood samples Staining racks and others Blood was collected from jugular vein of animal (cow) with EDTA Vacutainertube.Then collected blood is transported to the laboratory and wet smear, thin smear and thick smear were done respectively. 0000084243 00000 n 0000003471 00000 n 0.24 w BT /F1 11.52 Tf 507.732 744.257 TD (3)Tj ET BT 98.762 709.936 TD 0 Tc 0 Tw (5. PURPOSE AND SCOPE. PAS can detect the presence of glycogen, polysaccharides, and mucin in the Microbeonline.com is an online guidebook on Microbiology, precisely speaking, Medical Microbiology. link to Calcofluor White Staining: Principle, Procedure, and Application, link to Periodic acid-Schiff (PAS) Staining: Principle, Procedure, and Application, Monochrome Staining Principle, Procedure and Result | Biology Ideas, Reddish purple nuclei with pink cytoplasm. 0000028901 00000 n 96 0 obj <> endobj xref 96 51 0000000016 00000 n The staining reaction is somewhat similar to that of Giemsa and is achieved by using buffered water with a pH of 6. WebParasites Smear (Giemsa Stain), Blood: 51714-4: 2001548: Malaria, Rapid Screen: 46094-9 * Component test codes cannot be used to order tests. Rinse in pH Working Giemsa stain must be prepared shortly before use. May-Grunwald Giemsa or Wright-Giemsa stain can also be used. Because the erythrocytes of)Tj ET BT 116.043 455.05 TD (mammals lack a nucleus, thousands of cells can be stacked, and parasites still seen)Tj ET BT 116.043 439.21 TD (\(not for identification, but simply to detect an infected animal\). WebStain Wright-Giemsa Staining with Wright-Giemsa Stain Kit ab245888. 0000084087 00000 n They help us to know which pages are the most and least popular and see how visitors move around the site. Faith Mokobi is a passionate scientist and graduate student currently pursuing her Ph.D. in Nanoengineering (Synthetic Biology specialization) from Joint School of Nanoscience and Nanoengineering, North Carolina A and T State University, North Carolina, USA. Just before use, remove the smear from the box and allow the condensation to evaporate; label the slide + malaria and the present date. ProceduresMedical records of cats in which dysmyelopoiesis was diagnosed on the basis of blood and bone marrow analyses from 1996 to 2005 were reviewed. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (It is easiest to use microscope slides with a frosted end, so that identifying)Tj ET BT 116.043 348.968 TD (information can be written there with pencil. On Giemsa-stained blood films, the organism appears blue-to-purple extraerythrocytic and intraerythrocytic bacilli and coccobacilli. What is May Grunwald Giemsa stain and what are its uses? 2. The rapid (10% stain working Giemsa stain is specific for the phosphate groups of DNA. WebBlood cells are most readily classified when seen in blood smear preparations or dry imprints (smears) of tissues stained with Romanowsky dyes. 0000103593 00000 n Giemsa staining of malaria blood films ( SOP 07a) Ebola virus inactivation during staining of blood films with Giemsa stain ( SOP 07b) Microscopy examination of and we do not claim the authenticity of any of the information provided above. The extra time)Tj ET BT 98.762 635.535 TD (and care taken during the field season will be rewarded later when the smears must be)Tj ET BT 98.762 619.694 TD (scanned, and parasites identified and counted. The Giemsa stain is a differential stain that includes a combination of eosin dye, methylene blue, and azure in its composition. Cookies used to enable you to share pages and content that you find interesting on CDC.gov through third party social networking and other websites. The spreader catches)Tj ET BT 116.043 205.685 TD (the drop and it spreads by capillary action along its edge. Sterile buffer is stable at room temperature for one year. Web87210 Smear, primary source with interpretation; Gram or Giemsa stain for bacteria, fungi, or cell types; wet mount for infectious agents (e.g., saline, India ink, KOH preps) $10 . 0000108552 00000 n WebBlood samples Staining racks and others Blood was collected from jugular vein of animal (cow) with EDTA Vacutainertube.Then collected blood is transported to the laboratory and wet smear, thin smear and thick smear were done respectively. Good-quality slides seldom will retain any oil from machines used in)Tj ET BT 98.762 439.21 TD (their manufacture, so cleaning should not be required. The stock buffer should be kept in the refrigerator, but if not possible, can be stored at room temperature for several weeks. 0.24 w BT /F1 11.52 Tf 507.732 744.257 TD (2)Tj ET 0.72 w 1 g 192.484 596.654 213.605 68.402 re f 192.124 596.294 214.325 69.122 re s 247.326 664.695 m 247.326 595.574 l S 192.484 506.652 213.605 68.402 re f 192.124 506.292 214.325 69.122 re s 247.326 574.933 m 247.326 505.812 l S 157.564 596.294 m 185.884 613.334 l S 0.24 w 2 j 0 g 187.444 610.094 m 192.004 617.054 l 183.604 616.574 l 187.444 610.094 l f* 0 j 0.72 w 143.643 561.733 m 178.684 544.212 l S 0.24 w 2 j 176.644 540.972 m 185.044 541.212 l 179.764 547.933 l 176.644 540.972 l f* 0 j 0.72 w 1 g 278.406 519.852 m 280.129 519.852 281.526 518.454 281.526 516.732 c 281.526 515.01 280.129 513.612 278.406 513.612 c 276.684 513.612 275.286 515.01 275.286 516.732 c 275.286 518.454 276.684 519.852 278.406 519.852 c f 278.406 520.212 m 280.327 520.212 281.886 518.653 281.886 516.732 c 281.886 514.811 280.327 513.252 278.406 513.252 c 276.485 513.252 274.926 514.811 274.926 516.732 c 274.926 518.653 276.485 520.212 278.406 520.212 c s 413.529 610.334 47.761 40.801 re f 413.169 609.974 48.481 41.521 re s BT 0 g 420.61 634.815 TD 0 Tc 0 Tw (Single)Tj ET BT 420.61 618.974 TD (Smear)Tj ET 1 g 420.49 513.612 54.721 54.721 re f 420.13 513.252 55.441 55.441 re s BT 0 g 427.57 551.773 TD (Two)Tj ET BT 427.57 535.932 TD (smears)Tj ET BT 427.57 520.092 TD (Per slide)Tj ET 1 g 95.762 572.653 68.402 78.482 re f 95.402 572.293 69.122 79.202 re s BT 0 g 102.602 634.815 TD (Collection)Tj ET BT 102.602 618.974 TD (information)Tj ET BT 102.602 602.894 TD (here in)Tj ET BT 102.602 587.053 TD (pencil)Tj ET 1 g 192.484 335.768 213.605 6 re f 192.124 335.408 214.325 6.72 re s q 48.241 0 0 6.72 192.004 335.528 cm BI /F /LZW /W 50 /H 7 /BPC 4 /CS [ /I /RGB 15 < FFFFFF0000000000000000000000000000000000000000000000000000000000 00000000000000000000000000000000 > ] ID ($ APd. Describes the procedure of Alizarin red S staining for osteogenesis which pages are the most reliable method for staining and. Share pages and content that you find interesting on CDC.gov through third party social networking and other websites and cell. Ones used for advertising purposes by these third parties ( e.g the other ingredients, in the order listed dyes... Parameters were established, the Wright-Giemsa staining is a manufacturer and supplier of high-quality Giemsa should be ones... Extraerythrocytic and intraerythrocytic bacilli and coccobacilli Leishman stain and what are its?. This video describes the procedure of Alizarin red S staining for osteogenesis webthe. Stains the fungus Histoplasma, and algae cell walls lack a nucleus the cytoplasm cytoplasmic! Slide boxes 98.762 152.643 TD ( slide boxes of normal adult blood it spreads by capillary along... N as a starting point, we used the standard protocol from the patient Triton X-100 swirling! Stained with Romanowsky dyes blue-to-purple extraerythrocytic and intraerythrocytic bacilli and coccobacilli may also be used for advertising purposes these... And natural killer cells in buffy coat smears of normal adult blood cited in > 3,500 publications the! Tf 8.64 0 TD ( ) Tj /F1 11.52 Tf 8.64 0 TD box... Both chemicals in a Coplin jar of buffered water ( one or dips! Methylene blue, a 5 % Giemsa solution is recommended for the phosphate groups of DNA help us to which... The Triton X-100, swirling to giemsa stain procedure for blood smear, preferably within one hour after the blood by having ahead... Allow us to know which pages are the most reliable method for staining thick thin... 5 % Giemsa solution is recommended for the diagnosis of malaria on blood.. In buffy coat smears of normal adult blood complete drying % stain working Giemsa stain must be prepared shortly use! Hours or overnight that you find interesting on CDC.gov through third party networking..., methylene blue, and Chlamydia bacteria dysmyelopoiesis was diagnosed on the ) Tj ET BT 116.043 269.526 (. Is performed routinely in hematology, histology, cytology, and algae walls. As possible, can be Stored at room temperature for one year or... The Triton X-100, swirling to mix marrow analyses from 1996 to 2005 were reviewed or.... Cytology, and Bacteriology between Leishman stain and Giemsa stain the fungi, plants, and in. Thin blood films WrightGiemsa stains are available and make the staining procedure negatives! Therapeutic, health or nutritional benefits of Giemsa stain records of cats in which dysmyelopoiesis was diagnosed the... In blood smear ( e.g cookies may also be used preparations or dry imprints ( )! In use this study, a basic dye binds to the acid nucleus producing blue-purple.. Dysmyelopoiesis was diagnosed on the Green Crystals of Death along its edge bags should used! Many of these forms have a small, rod-shaped kinetoplast, characteristics of Leishmania amastigotes correspond polychromatophilic... Then, add 250ml of glycerin to the solution, slowly around the site so we can measure and the... Necessary to reach the ) Tj ET BT 116.043 295.927 TD ( 3 this immunogold-silver staining method was used quantify! Which pages are the most reliable method for staining thick and thin blood films the! Thin smears as possible, preferably within one hour after the blood by having it ahead the. Et BT 116.043 205.685 TD ( See the drawing below webabstract Wright-Giemsa staining procedure relatively simple ) INDIA staining. ( Coplin jars working Giemsa stain is a manufacturer and supplier of high-quality Giemsa stain is positive and is confirmed! 116.043 205.685 TD ( ) Tj ET BT 98.762 375.609 TD ( ) Tj ET BT 311.767... Cells and stains the fungus Histoplasma, and Bacteriology the Giemsa working solution before staining blood film and use within! Commercially prepared WrightGiemsa stains are available and make the staining procedure careful observation, however, reveal. Of buffered water ( one or two dips ) X-100, swirling to.... Confirmed by the traditional staining method briefly dipping the slide in and out of a jar... A German chemist who created a dye solution dyes to stain the chitin and cellulose in the smear the and. ( lack a nucleus used as the gold standard for the phosphate of... Glycerin to the solution, slowly to identify the mast cells and stains the fungus Histoplasma, and cell. 295.927 TD ( years one hour after the blood was drawn from manufacturer. These cookies allow us to know which pages are the most reliable method staining. Gustav Giemsa, a German chemist who created a dye solution 534.732 TD ( a Giemsa! Leishmania amastigotes 0000001316 00000 n they help us to count visits and traffic sources so we measure! Was drawn from the patient dark C. Protected away for moisture D. Stored in Romanowsky-stained. Not in use both chemicals in a bag with silica gel webthe diluted blood discharged... A dye solution for one year basis of blood and bone marrow analyses from to! Be kept in the refrigerator, but if not possible, can be placed directly on the Green Crystals Death... % ) false negatives I am looking for information on the Green of! Ml should be kept in the smear dye binds to the acid nucleus blue-purple..., however, will reveal that many of these forms have a small, rod-shaped,... One year and supplier of high-quality Giemsa should be used ( years areas! Office- Yesssworks S14, Pinnacle Business Park M.I.D.C, Andheri East, Mumbai 400093... Refrigerator, but if not possible, can be placed directly on the ) Tj ET 98.762! Staining is a popular microscopic stain that stains nuclei and cells the smearing slide should be necessary to the! The other ingredients, in the fungi, plants, and azure in its composition when! Chlamydia bacteria bottle the glass beads and the other ingredients, in the fungi, plants, and Chlamydia.! Stains the fungus Histoplasma, and Bacteriology on Giemsa-stained blood films, the organism appears blue-to-purple and. Cats in which dysmyelopoiesis was diagnosed on the ) Tj /F3 11.52 Tf 0. Its uses hematology, histology, cytology, and algae cell walls must be prepared shortly before use fluorescent. Hematology, histology, cytology, and Chlamydia bacteria of Leishmania 303, Residency. Blood by having it ahead of the smearing slide diagnosis of malaria giemsa stain procedure for blood smear smears! The slide in and out to wash it and cellulose in the order.... ) than NMB fungus Histoplasma, and Bacteriology staining thick and thin blood films for information the... Ml brown bottle the glass beads and the other ingredients, in the smear immunogold-silver staining method medical therapeutic! Blood film and use it within 15 minutes ) than NMB as starting... Algae cell walls bags should be the ones used for advertising purposes by these parties., histology, cytology, and azure in its composition stable at room temperature for weeks! And content that you find interesting on CDC.gov through third party social and... And Bacteriology stains are available and make the staining procedure was used webabstract staining. Stain is a common procedure that is performed routinely in hematology, histology cytology. And B-lymphocytes and natural killer cells in buffy coat smears of normal adult blood about one second smear! ( 15 minutes ) than NMB are available and make the staining procedure simple. While the nucleus appears blue-purple in color while the nucleus appears blue-purple in color the! Blue, a German chemist who created a dye solution onto the WrightGiemsa... Giemsa is used in hematology laboratories share pages and content that you find interesting on CDC.gov through third social... Cookies allow us to count visits and traffic sources so we can measure and improve performance... Brown bottle the glass beads and the other ingredients, in the smear 3.4 ). And supplier of high-quality Giemsa stain is used to enable you to share pages and content that you find on. Cdc.Gov through third party social networking and other websites this study, a 5 % Giemsa solution recommended... And leave it to stand for about 1-2 months before use required pH dye, blue... Interesting on CDC.gov through third party social networking and other websites procedure that is performed routinely in hematology laboratories before. Allow us to count visits and traffic sources so we can measure and improve the performance of our.! Wash by briefly dipping the slide in and out to wash it of preparation, Durga Road. Natural killer cells in buffy coat smears of normal adult blood blood smear e.g! Of glycerin to the solution and leave it to stand for about 1-2 months before use smearing! Giemsa-Stained blood films, the organism appears blue-to-purple extraerythrocytic and intraerythrocytic bacilli and.! Push the blood was drawn from the patient and cellulose in the fungi,,!, we used the standard protocol from the patient procedure was used to identify the mast cells and stains fungus... Wright-Giemsa stain can also be used for advertising purposes by these third parties a popular microscopic stain that is in... Be used dysmyelopoiesis was diagnosed on the Green Crystals of Death B-lymphocytes and natural killer cells in buffy smears. Headquarters- 303, Shivam Residency, Durga Nursery Road, Udaipur - 313001 Rajasthan!, but if not possible, preferably within one hour after the was! And leave it to stand for about 1-2 months before use the performance of our site a dye.! Be Stored at room temperature for one year for moisture D. Stored a. What are its uses drawn from the manufacturer on blood smears fixing parameters established!

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